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sb 505124  (Tocris)


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    Structured Review

    Tocris sb 505124
    Sb 505124, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sb+505124/SB+505124/bio_rxiv__64898__2026__04__21__719911-172-12-13
    Average 95 stars, based on 59 article reviews
    sb 505124 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: The role of DNA methylation in chondrogenesis of human iPSCs as a stable marker of cartilage quality
    Article Snippet: Before induction of anterior primitive streak (day 0), hiPSCs were washed with wash medium (IMDM/F12 and 0.5% P/S) and then fed with MD medium supplemented with activin A (30 ng/ml; Stemgent), 4 μM CHIR99021 (CHIR; Stemgent), and human fibroblast growth factor (20 ng/ml; FGF-2; R&D Systems) for 24 h. Subsequently, the cells were washed again with wash medium, and paraxial mesoderm was induced on day 1, by MD medium supplemented with 2 μM SB-505124 (Tocris), 3 μM CHIR, FGF-2 (20 ng/ml), and 4 μM dorsomorphin (Tocris) for 24 h. Before induction of early somite (day 2), cells were washed with wash medium, and then, cells were fed with MD medium supplemented with 2 μM SB-505124, 4 μM dorsomorphin, 1 μM C59 (Cellagen Technology), and 500 nM PD173074 (Tocris) for 24 h. Subsequently, cells were washed with wash medium, and for induction of sclerotome, cells (days 3 to 5) were fed daily with MD medium supplemented with 2 μM purmorphamine (Stemgent) and 1 μM C59.

    Article Title: Isolation and tracing of matrix-producing notochordal and chondrocyte cells using ACAN-2A-mScarlet reporter human iPSC lines
    Article Snippet: For induction of paraxial mesoderm (day 1), cells were briefly washed with the wash medium and were fed with the mesodermal differentiation medium supplemented with 2 μM SB-505124 (SB5; Tocris, no. 3263), 3 μM CHIR99021, FGF (20 ng/ml), and 4 μM dorsomorphin (DM; Stemgent, no. 04-0024) for 24 hours.

    Article Title: The role of DNA methylation in chondrogenesis of human iPSCs as a stable marker of cartilage quality.
    Article Snippet: Before induction of anterior primitive streak (day 0), hiPSCs A Compare cell sources were washed with wash medium (IMDM/F12 and 0.5% P/S) and then fed with MD medium supplemented with activin A (30 ng/ml; Stemgent), 4 μM CHIR99021 (CHIR; Stemgent), and human fibroblast growth factor (20 ng/ ml; FGF-2; R&D Systems) for 24 h. Subsequently, the cells were washed again with wash medium, and paraxial mesoderm was induced on day 1, by MD medium supplemented with 2 μM SB-505124 (Tocris), 3 μM CHIR, FGF-2 (20 ng/ml), and 4 μM dorsomorphin (Tocris) for 24 h. Before induction of early somite (day 2), cells were washed with wash medium, and then, cells were fed with MD medium supplemented with 2 μM SB-505124, 4 μM dorsomorphin, 1 μM C59 (Cellagen Technology), and 500 nM PD173074 (Tocris) for 24 h. Subsequently, cells were washed with wash medium, and for induction of sclerotome, cells (days 3 to 5) were fed daily with MD medium supplemented with 2 μM purmorphamine (Stemgent) and 1 μM C59.

    Recombinant:

    Article Title: Nuclear Factor I genes drive chondrogenic cell-fate commitment
    Article Snippet: On Day 0, basal medium was supplemented with 30ng/ml Activin A (Miltenyibiotec), 4μM of CHIR99021 (Tocris), and 20ng/ml recombinant human fibroblast growth factor (basic, Peprotech). .. On day 1, cells were refreshed with basal medium supplemented with 2μM SB-505124 (Tocris), 3μM CHIR99021, 20 ng/ml recombinant human fibroblast growth factor, 4μM Dorsomorphin dihydrochloride (Tocris). .. On day 2 basal medium was supplemented with 2μM SB-505124, 1μM Wnt-C59 (Tocris), 500nM PD173074 (Tocris), and 4μM Dorsomorphin.



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